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siha id 60528  (BioResource International Inc)
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BioResource International Inc siha id 60528
Effects of L-AA on other human cervical cancer cell lines. C33A (A) and <t>SiHa</t> (B) cells (2 × 10 5 cells/well) were incubated for 24 h with the indicated concentrations of L-AA. (C,D) HeLa cells (2 × 10 5 cells/well) were incubated for 24 h with the indicated concentrations of (C) sodium ascorbate or (D) indicated reagents. Cell lysates were subjected to Western blot analysis using antibodies against Nrf2, p62, e-IF2α, and p-e-IF2α. ACTN was the protein loading control. The results are representative of three independent experiments. Protein bands were quantified through pixel density scanning and evaluated using ImageJ, version 1.44a ( http://imagej.nih.gov/ij/ ). The fold was normalized to the internal control protein (ACTN).
Siha Id 60528, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effects of L-AA on other human cervical cancer cell lines. C33A (A) and SiHa (B) cells (2 × 10 5 cells/well) were incubated for 24 h with the indicated concentrations of L-AA. (C,D) HeLa cells (2 × 10 5 cells/well) were incubated for 24 h with the indicated concentrations of (C) sodium ascorbate or (D) indicated reagents. Cell lysates were subjected to Western blot analysis using antibodies against Nrf2, p62, e-IF2α, and p-e-IF2α. ACTN was the protein loading control. The results are representative of three independent experiments. Protein bands were quantified through pixel density scanning and evaluated using ImageJ, version 1.44a ( http://imagej.nih.gov/ij/ ). The fold was normalized to the internal control protein (ACTN).

Journal: Frontiers in Oncology

Article Title: Mechanisms and Applications of the Anti-cancer Effect of Pharmacological Ascorbic Acid in Cervical Cancer Cells

doi: 10.3389/fonc.2020.01483

Figure Lengend Snippet: Effects of L-AA on other human cervical cancer cell lines. C33A (A) and SiHa (B) cells (2 × 10 5 cells/well) were incubated for 24 h with the indicated concentrations of L-AA. (C,D) HeLa cells (2 × 10 5 cells/well) were incubated for 24 h with the indicated concentrations of (C) sodium ascorbate or (D) indicated reagents. Cell lysates were subjected to Western blot analysis using antibodies against Nrf2, p62, e-IF2α, and p-e-IF2α. ACTN was the protein loading control. The results are representative of three independent experiments. Protein bands were quantified through pixel density scanning and evaluated using ImageJ, version 1.44a ( http://imagej.nih.gov/ij/ ). The fold was normalized to the internal control protein (ACTN).

Article Snippet: HeLa cervical carcinoma (ID 60005), C33A (ID 60554), and SiHa (ID 60528) cells were purchased from Bioresource Collection and Research Center, Taiwan, Republic of China.

Techniques: Incubation, Western Blot, Control